Fort Niobrara National Wildlife Refuge NWR is 19,131 acres in size and located 4 miles east of Valentine along the Niobrara River in north-central Nebraska. Fort Niobrara NWR was established by Executive Order in January 1912 as a preserve and breeding ground for native birds. Its purpose was expanded later that same year to include the preservation of bison and elk herds representative of those that once roamed the Great Plains.

The rolling sandhills and breaks along the Niobrara River canyon are home to a great variety of wildlife. In the winter, bald and golden eagles can be seen along the river. Wild turkeys are spotted among the birch, burr oak, and Ponderosa pine. The unusual and unique assemblage of plant communities currently present on the Refuge, including sandhills prairie, mixed prairie, Rocky Mountain coniferous forest, eastern deciduous forest, and northern boreal forest, supports a rich diversity of wildlife largely unchanged from pre-settlement times.

The Refuge offers many activities for visitors. Bison, elk, deer, and prairie dogs can be seen along the wildlife drive at all times of the year. Interpretive displays at the visitor center explore the history and ecology of the area. Hiking trails lead to Fort Falls and, for the more adventurous, into the Niobrara River Wilderness Area. Canoeing or tubing through the Refuge down the Niobrara River is a popular activity.